Considerable efforts have been made to synthesize and characterize protein-nanoparticle conjugates (protein-NPs) for their promising applications in bionanotechnology. However, protein concentration determination in the protein-NPs has so far not been reported. In this letter, we present a simple and non-destructive approach to quantify the protein concentration in the protein-NPs aqueous solution using circular dichroism (CD) spectroscopy. Carbon dots (~4 nm), gold nanoparticles (~10 nm) and polyethylene glycol (PEG, molecular weight ~3000) were either physically mixed or covalently conjugated (not in the case of gold nanoparticles) with proteins (human transferrin, human serum albumin, and ovalbumin). We were able to quantify the protein concentration in the protein-nanoparticle conjugates using a calibration curve from the CD spectra.
Pharmacology, Toxicology and Pharmaceutical Science
8
5%
Other
14
9%
Unknown
51
33%
Attention Score in Context
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 October 2019.
All research outputs
#17,855,227
of 27,798,941 outputs
Outputs from Analytical Chemistry
#21,036
of 29,726 outputs
Outputs of similar age
#145,793
of 266,685 outputs
Outputs of similar age from Analytical Chemistry
#174
of 311 outputs
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We're also able to compare this research output to 311 others from the same source and published within six weeks on either side of this one. This one is in the 41st percentile – i.e., 41% of its contemporaries scored the same or lower than it.
