| Title |
Assaying genome-wide recombination and centromere functions with Arabidopsis tetrads
|
|---|---|
| Published in |
Proceedings of the National Academy of Sciences of the United States of America, January 1998
|
| DOI | 10.1073/pnas.95.1.247 |
| Pubmed ID | |
| Authors | |
| Abstract |
During meiosis, crossover events generate new allelic combinations, yet the abundance of these genetic exchanges in individual cells has not been measured previously on a genomic level. To perform a genome-wide analysis of recombination, we monitored the assortment of genetic markers in meiotic tetrads from Arabidopsis. By determining the number and distribution of crossovers in individual meiotic cells, we demonstrated (i) surprisingly precise regulation of crossover number in each meiosis, (ii) considerably reduced recombination along chromosomes carrying ribosomal DNA arrays, and (iii) an inversely proportional relationship between recombination frequencies and chromosome size. This use of tetrad analysis also achieved precise mapping of all five Arabidopsis centromeres, localizing centromere functions in the intact chromosomes of a higher eukaryote. |
Login to access the Attention Digest and the Sentiment Analysis related to this output.
Mendeley demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 5 | 4% |
| Germany | 1 | <1% |
| Australia | 1 | <1% |
| Unknown | 123 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 42 | 32% |
| Student > Ph. D. Student | 24 | 18% |
| Professor | 14 | 11% |
| Professor > Associate Professor | 10 | 8% |
| Student > Doctoral Student | 6 | 5% |
| Other | 14 | 11% |
| Unknown | 20 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 82 | 63% |
| Biochemistry, Genetics and Molecular Biology | 21 | 16% |
| Philosophy | 1 | <1% |
| Immunology and Microbiology | 1 | <1% |
| Physics and Astronomy | 1 | <1% |
| Other | 4 | 3% |
| Unknown | 20 | 15% |