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Timeline
Attention over time
Attention Score history
Mendeley readers
| Title |
Affinity Purification of a Human RNA Polymerase II Complex Using Monoclonal Antibodies against Transcription Factor IIF*
|
|---|---|
| Published in |
Journal of Biological Chemistry, April 1997
|
| DOI | 10.1074/jbc.272.17.11495 |
| Pubmed ID | |
| Authors | |
| Abstract |
Five different monoclonal antibodies that immunoreact with RAP74, the large subunit of general transcription factor (TF) IIF, were produced and characterized. Using one of these antibodies, an affinity purification procedure was devised to isolate a human RNA polymerase II complex. This procedure is fast, simple, and reproducible and does not require extensive purification. The RNA polymerase II complex isolated using this procedure contains SRB (suppressor of RNA polymerase B) polypeptides, transcription factors IIE and IIF, limiting amounts of TFIIH, and the TATA-binding protein, but was devoid of TFIIB. |
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Mendeley readers
The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 13 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 38% |
| Student > Ph. D. Student | 2 | 15% |
| Professor > Associate Professor | 2 | 15% |
| Other | 1 | 8% |
| Student > Bachelor | 1 | 8% |
| Other | 1 | 8% |
| Unknown | 1 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 8 | 62% |
| Biochemistry, Genetics and Molecular Biology | 3 | 23% |
| Computer Science | 1 | 8% |
| Unknown | 1 | 8% |