Title |
Draft genome sequence and characterization of Desulfitobacterium hafniense PCE-S
|
---|---|
Published in |
Environmental Microbiome, February 2015
|
DOI | 10.1186/1944-3277-10-15 |
Pubmed ID | |
Authors |
Tobias Goris, Bastian Hornung, Thomas Kruse, Anika Reinhold, Martin Westermann, Peter J Schaap, Hauke Smidt, Gabriele Diekert |
Abstract |
This genome report describes the draft genome and the physiological characteristics of Desulfitobacterium hafniense PCE-S, a Gram-positive bacterium known to dechlorinate tetrachloroethene (PCE) to dichloroethene by a PCE reductive dehalogenase. The draft genome has a size of 5,666,696 bp with a G + C content of 47.3%. The genome is very similar to the already sequenced Desulfitobacterium hafniense Y51 and the type strain DCB-2. We identified two complete reductive dehalogenase (rdh) genes in the genome of D. hafniense PCE-S, one of which encodes PceA, the PCE reductive dehalogenase, and is located on a transposon. Interestingly, this transposon structure differs from the PceA-containing transposon of D. hafniense Y51. The second rdh encodes an unknown reductive dehalogenase, highly similar to rdhA 7 found in D. hafniense DCB-2, in which the corresponding gene is disrupted. This reductive dehalogenase might be responsible for the reductive dechlorination of 2,4,5-trichlorophenol and pentachlorophenol, which is mediated by D. hafniense PCE-S in addition to the reductive dechlorination of PCE. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Germany | 1 | 4% |
Unknown | 27 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 10 | 36% |
Student > Bachelor | 4 | 14% |
Professor > Associate Professor | 3 | 11% |
Student > Doctoral Student | 2 | 7% |
Researcher | 2 | 7% |
Other | 5 | 18% |
Unknown | 2 | 7% |
Readers by discipline | Count | As % |
---|---|---|
Environmental Science | 5 | 18% |
Agricultural and Biological Sciences | 5 | 18% |
Engineering | 4 | 14% |
Biochemistry, Genetics and Molecular Biology | 4 | 14% |
Nursing and Health Professions | 1 | 4% |
Other | 6 | 21% |
Unknown | 3 | 11% |