In this study, we primarily sought to assess the ability of flow cytometry to predict early clinical deterioration and overall survival in septic patients admitted in the emergency department and intensive care unit.
Patients admitted for community-acquired acute sepsis from 11 hospital centers were eligible. Early (Day 7) and late (Day 28) deaths were notified. Levels of CD64pos granulocytes, CD16pos monocytes, CD16dim immature granulocytes (IG), T and B lymphocytes were assessed by flow cytometry, using an identical, cross-validated, robust and simple consensus standardized protocol in each center.
Among 1062 patients screened, 781 patients with confirmed sepsis were studied (age: 67±48 years, SAPS II: 36±17, SOFA: 5±4). Patients were divided into three groups (sepsis, severe sepsis and septic shock) on Day 0 and on Day 2. On Day 0, septic patients exhibited increased level of CD64pos granulocytes, CD16pos monocytes and IG with T-cell lymphopenia. Clinical severity was associated with higher percentages of IG and deeper T-cell lymphopenia. IG percentages tended to be higher in patients whose clinical status worsened on Day 2 (35.1 ± 35.6 vs 43.5 ± 35.2, p=0.07). Increased IG percentages were also related to occurrence of new organ failures on Day 2. Increased IG percentages, especially when associated with T-cell lymphopenia, were independently associated with early (p<0.01) and late (p<0.01) death.
Increased circulating IG at the acute phase of sepsis are linked to clinical worsening, especially when associated with T-cell lymphopenia. Early flow cytometry could help clinicians to target patients at high risk of clinical deterioration.
Pharmacology, Toxicology and Pharmaceutical Science
2
2%
Other
7
8%
Unknown
36
41%
Attention Score in Context
Attention Score in Context
This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 September 2018.
All research outputs
#10,670,867
of 34,411,289 outputs
Outputs from CHEST
#9,210
of 18,094 outputs
Outputs of similar age
#133,082
of 370,441 outputs
Outputs of similar age from CHEST
#56
of 109 outputs
Altmetric has tracked 34,411,289 research outputs across all sources so far. This one has received more attention than most of these and is in the 68th percentile.
So far Altmetric has tracked 18,094 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 13.1. This one is in the 49th percentile – i.e., 49% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 370,441 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 63% of its contemporaries.
We're also able to compare this research output to 109 others from the same source and published within six weeks on either side of this one. This one is in the 48th percentile – i.e., 48% of its contemporaries scored the same or lower than it.
