Transcription factor AP-2 plays a critical role in regulating gene expression during vertebrate development and cellular differentiation. We report here that AP-2 DNA binding in vitro can be reversibly modulated by redox conditions and that thioredoxin is a potent stimulator of AP-2 DNA binding. Our studies indicate that oxidation of recombinant human AP-2 with diamide or hydrogen peroxide inhibits its DNA binding activity to synthetic AP-2 oligodeoxynucleotides. The inhibitory effect of diamide on AP-2 DNA binding was dose-dependent and was reversible by addition of reducing agents beta-mercaptoethanol, dithiothreitol, and thioredoxin. Immunoblot assays indicated that treatment with oxidants caused a change in migration of the AP-2 protein in nonreducing gels and that this effect was also reversible by treatment of the oxidized AP-2 with reducing agents. These data suggest a mechanism by which the oxidation state of conserved cysteine residues in the AP-2 DNA binding domain may contribute to its DNA binding activity. AP-2 joins a group of other transcription factors whose functions are regulated in part by their redox states.
Pharmacology, Toxicology and Pharmaceutical Science
1
9%
Biochemistry, Genetics and Molecular Biology
1
9%
Unknown
1
9%
Attention Score in Context
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 August 2018.
All research outputs
#28,600,579
of 34,359,530 outputs
Outputs from Biochemical & Biophysical Research Communications
#28,507
of 33,075 outputs
Outputs of similar age
#44,223
of 46,069 outputs
Outputs of similar age from Biochemical & Biophysical Research Communications
#224
of 227 outputs
Altmetric has tracked 34,359,530 research outputs across all sources so far. This one is in the 9th percentile – i.e., 9% of other outputs scored the same or lower than it.
So far Altmetric has tracked 33,075 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.2. This one is in the 8th percentile – i.e., 8% of its peers scored the same or lower than it.
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We're also able to compare this research output to 227 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
