Genetic analysis of water loss of excised leaves associated with drought tolerance in wheat

Ilona Mieczysława Czyczyło-Mysza, Izabela Marcińska, Edyta Skrzypek, Jan Bocianowski, Kinga Dziurka, Dragana Rančić, Radenko Radošević, Sofija Pekić-Quarrie, Dejan Dodig, Stephen Alexander Quarrie

Wheat is widely affected by drought. Low excised-leaf water loss (ELWL) has frequently been associated with improved grain yield under drought. This study dissected the genetic control of ELWL in wheat, associated physiological, morphological and anatomical leaf traits, and compared these with yield QTLs. Ninety-four hexaploid wheat (Triticum aestivum L.) doubled haploids, mapped with over 700 markers, were tested for three years for ELWL from detached leaf 4 of glasshouse-grown plants. In one experiment, stomata per unit area and leaf thickness parameters from leaf cross-sections were measured. QTLs were identified using QTLCartographer. ELWL was significantly negatively correlated with leaf length, width, area and thickness. Major QTLs for ELWL during 0-3 h and 3-6 h were coincident across trials on 3A, 3B, 4B, 5B, 5D, 6B, 7A, 7B, 7D and frequently coincident (inversely) with leaf size QTLs. Yield in other trials was sometimes associated with ELWL and leaf size phenotypically and genotypically, but more frequently under non-droughted than droughted conditions. QTL coincidence showed only ELWL to be associated with drought/control yield ratio. Our results demonstrated that measures of ELWL and leaf size were equally effective predictors of yield, and both were more useful for selecting under favourable than stressed conditions.