This study examined acute and chronic changes in perceptual measures (rating of perceived exertion (RPE), affect, and arousal) in response to two regimens of high intensity interval training (HIIT). Twenty three healthy sedentary women (mean ± SD age and VO2max = 23.0 ± 5.7 yr and 30.1 ± 4.4 mL.kg.-1.min-1, respectively) were randomized to complete 12 wks of one of two HIIT regimes, while an additional seven women served as sedentary controls. Training was performed 3 d.wk-1 on a cycle ergometer and consisted of up to ten 1 min bouts at moderate (60 - 80 %Wmax = MOD) or more intense (80 - 90 %Wmax = HI) workloads separated by active recovery. At baseline and every 3 wks, RPE, affect, and arousal were measured during training using validated scales. Repeated measures analysis of variance was used to examine acute and chronic changes in these variables to HIIT. Data revealed significant (p < 0.001) increases in RPE and arousal and decreases (p < 0.001) in affect during acute HIIT, with RPE responses differing (p < 0.05) between HI and MOD. However, acute changes in affect and arousal were similar in HI and MOD. Training led to a significant reduction in RPE; whereas, both affect and arousal were unchanged (p > 0.05) after HIIT. Completion of moderate or more intense interval training reduces perceptions of RPE during training, yet does not alter arousal or affect. RPE was reduced via training, yet large dependence upon anaerobic metabolism during HIIT may minimize training-induced changes in affect.