Comparative antioxidative analyses of aqueous ethanolic extracts from leaf, root, and stem of G. buchananii revealed high activity of all three organs. To investigate the metabolites composition of the different parts of G. buchananii, an untargeted metabolomics approach using UPLC-ESI-TOF MS with simultaneous acquisition of low- and high-collision energy mass spectra (MS(e)) was performed. Unsupervised statistics (PCA) highlighted clear differences in the metabolomes of the three organs. OPLS-DA revealed (2R,3S,2''R,3''R)-GB-1, (2R,3S)-morelloflavone, and (2R,3S)-volkensiflavone as the most decisive marker compounds discriminating leaf from root and stem extract. Leaves represent the best source to isolate GB-1, morelloflavone, and volkensiflavone. Root extract is the best organ to isolate xanthones, stem bark extract the best source to isolate (2R,3S,2''R,3''R)-manniflavanone, and the identified polyisoprenylated benzophenones are characteristic compounds for the leaf organ. Morelloflavone, volkensiflavone and garcicowin C were isolated for the first time from G. buchananii and identified via MS, NMR, and CD spectroscopy and showed in H2O2 scavenging, H/L-TEAC, and H/L-ORAC assays moderate to strong in vitro antioxidative activities.
Pharmacology, Toxicology and Pharmaceutical Science
4
10%
Chemical Engineering
2
5%
Physics and Astronomy
2
5%
Other
4
10%
Unknown
11
28%
Attention Score in Context
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 September 2015.
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#24,756,754
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Outputs of similar age from Journal of Agricultural & Food Chemistry
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