Title |
Ruthenium(II) p-cymene complex bearing 2,2′-dipyridylamine targets caspase 3 deficient MCF-7 breast cancer cells without disruption of antitumor immune response
|
---|---|
Published in |
Journal of Inorganic Biochemistry, September 2015
|
DOI | 10.1016/j.jinorgbio.2015.09.006 |
Pubmed ID | |
Authors |
Goran N Kaluđerović, Tamara Krajnović, Miljana Momcilovic, Stanislava Stosic-Grujicic, Sanja Mijatović, Danijela Maksimović-Ivanić, Evamarie Hey-Hawkins |
Abstract |
[Ru(η(6)-p-cym)Cl{dpa(CH2)4COOEt}][PF6] (cym=cymene; dpa=2,2'-dipyridylamine; complex 2) was prepared and characterized by elemental analysis, IR and multinuclear NMR spectroscopy, as well as ESI-MS and X-ray structural analysis. The structural analog without a side chain [Ru(η(6)-p-cym)Cl(dpa)][PF6] (1) as well as 2 were investigated in vitro against 518A2, SW480, 8505C, A253 and MCF-7 cell lines. Complex 1 is active against all investigated tumor cell lines while the activity of compound 2 is limited only to caspase 3 deficient MCF-7 breast cancer cells, however, both are less active than cisplatin. As CD4(+)Th cells are necessary to trigger all the immune effector mechanisms required to eliminate tumor cells, besides testing the in vitro antitumor activity of 1 and 2, the effect of ruthenium(II) complexes on the cells of the adaptive immune system have also been evaluated. Importantly, complex 1 applied in concentrations which were effective against tumor cells did not affect immune cell viability, nor did exert a general immunosuppressive effect on cytokine production. Thus, beneficial characteristics of 1 might contribute to the overall therapeutic properties of the complex. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 2% |
Serbia | 1 | 2% |
Unknown | 49 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 9 | 18% |
Researcher | 7 | 14% |
Student > Ph. D. Student | 7 | 14% |
Student > Doctoral Student | 5 | 10% |
Student > Bachelor | 4 | 8% |
Other | 12 | 24% |
Unknown | 7 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Chemistry | 20 | 39% |
Biochemistry, Genetics and Molecular Biology | 8 | 16% |
Agricultural and Biological Sciences | 4 | 8% |
Immunology and Microbiology | 2 | 4% |
Computer Science | 2 | 4% |
Other | 6 | 12% |
Unknown | 9 | 18% |