Title |
PKS Activities and Biosynthesis of Cannabinoids and Flavonoids in Cannabis sativa L. Plants
|
---|---|
Published in |
Plant & Cell Physiology, December 2008
|
DOI | 10.1093/pcp/pcn150 |
Pubmed ID | |
Authors |
Isvett Josefina Flores-Sanchez, Robert Verpoorte |
Abstract |
Polyketide synthase (PKS) enzymatic activities were analyzed in crude protein extracts from cannabis plant tissues. Chalcone synthase (CHS, EC 2.3.1.74), stilbene synthase (STS, EC 2.3.1.95), phlorisovalerophenone synthase (VPS, EC 2.3.1.156), isobutyrophenone synthase (BUS) and olivetol synthase activities were detected during the development and growth of glandular trichomes on bracts. Cannabinoid biosynthesis and accumulation take place in these glandular trichomes. In the biosynthesis of the first precursor of cannabinoids, olivetolic acid, a PKS could be involved; however, no activity for an olivetolic acid-forming PKS was detected. Content analyses of cannabinoids and flavonoids, two secondary metabolites present in this plant, from plant tissues revealed differences in their distribution, suggesting a diverse regulatory control for these biosynthetic fluxes in the plant. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 2 | <1% |
Brazil | 2 | <1% |
Canada | 2 | <1% |
Germany | 1 | <1% |
Unknown | 225 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 59 | 25% |
Student > Ph. D. Student | 29 | 13% |
Student > Bachelor | 28 | 12% |
Student > Master | 27 | 12% |
Other | 10 | 4% |
Other | 31 | 13% |
Unknown | 48 | 21% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 81 | 35% |
Biochemistry, Genetics and Molecular Biology | 36 | 16% |
Chemistry | 22 | 9% |
Pharmacology, Toxicology and Pharmaceutical Science | 16 | 7% |
Chemical Engineering | 7 | 3% |
Other | 19 | 8% |
Unknown | 51 | 22% |