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Molecular Characterization of Multidrug-Resistant Escherichia coli Isolates from Bovine Clinical Mastitis and Pigs in the Vojvodina Province, Serbia

Overview of attention for article published in Microbial Drug Resistance: Mechanism, Epidemiology, & Disease, May 2017
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Title
Molecular Characterization of Multidrug-Resistant Escherichia coli Isolates from Bovine Clinical Mastitis and Pigs in the Vojvodina Province, Serbia
Published in
Microbial Drug Resistance: Mechanism, Epidemiology, & Disease, May 2017
DOI 10.1089/mdr.2017.0016
Pubmed ID
Authors

Dalibor Todorović, Maja Velhner, Edita Grego, Dejan Vidanović, Dubravka Milanov, Dejan Krnjaić, Corinna Kehrenberg

Abstract

The aim of the study was to characterize multidrug-resistant (MDR) Escherichia coli isolates collected in Serbia from bovine clinical mastitis cases and diseased pigs, mainly with molecular methods. A total of 48 E. coli isolates was collected during the years 2013-2014, of which 22 were MDR and were included in further analysis. Phylogenetic typing showed that 17 isolates belonged to group A, while two isolates were classified in group B1 and a single one in group D. All isolates showed unique macrorestriction patterns. Phenotypic susceptibility testing revealed resistances of the isolates against up to 13 antimicrobial agents, including resistance to fluoroquinolones. A wide variety of resistance genes was detected by PCR amplification and sequencing of amplicons. Sequence analysis of the quinolone resistance determining regions of topoisomerase genes revealed mutations in gyrA, parC, and/or parE. Plasmid-mediated quinolone resistance genes were detected in two porcine (aac-6'-Ib-cr and qnrS, respectively) isolates and a single bovine (aac-6'-Ib-cr) isolate. Resistance genes were found to be located on conjugative plasmids in 16 cases, many of which conferred a multidrug resistance phenotype. In conclusion, the plentitude of resistance genes located on conjugative plasmids and integrons in E. coli from cows and pigs in Vojvodina, Serbia, pose a high risk for horizontal gene transfer in bacteria from livestock husbandry.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 47 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 47 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 8 17%
Researcher 7 15%
Student > Master 5 11%
Student > Doctoral Student 4 9%
Student > Bachelor 3 6%
Other 5 11%
Unknown 15 32%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 9 19%
Agricultural and Biological Sciences 7 15%
Immunology and Microbiology 4 9%
Medicine and Dentistry 3 6%
Biochemistry, Genetics and Molecular Biology 2 4%
Other 4 9%
Unknown 18 38%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 August 2018.
All research outputs
#20,660,571
of 25,382,440 outputs
Outputs from Microbial Drug Resistance: Mechanism, Epidemiology, & Disease
#702
of 1,191 outputs
Outputs of similar age
#251,666
of 326,979 outputs
Outputs of similar age from Microbial Drug Resistance: Mechanism, Epidemiology, & Disease
#18
of 30 outputs
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