Advanced in vitro cervical tumor model was established by 3D printing to study the epithelial-to-mesenchymal transition (EMT), which is a very important stage of dissemination of carcinoma leading to metastatic tumors. A Hela/hydrogel grid construct composed of gelatin, alginate, matrigel and Hela cells was fabricated by forced extrusion in a layer-by-layer fashion. Hela cells rapidly proliferated, formed spheroids and presented tumorigenic characteristic in the 3D-printed structure. With the supplement of TGF-β, aggregated Hela cells started to disintegrate, and some of them changed into fibroblast-like spindle morphology, which indicate epithelial-to-mesenchymal transition was induced. The down-regulation of epithelial marker E-cadherin, and up-regulation of mesenchymal markers like snail, vimentin and N-cadherin were all observed in 3D-printed model, and performed differently in 3D and 2D models. The TGF-β induced EMT was inhibited by the treatment of disulfiram and EMT pathway inhibitor C19 in a dose dependent manner, showing a great potential in future study of therapeutic program towards cervical tumor metastasis.
This research output has an Altmetric Attention Score of 19. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 October 2023.
All research outputs
#2,402,583
of 34,089,221 outputs
Outputs from Biofabrication
#129
of 1,000 outputs
Outputs of similar age
#37,624
of 377,636 outputs
Outputs of similar age from Biofabrication
#3
of 9 outputs
Altmetric has tracked 34,089,221 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 92nd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,000 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 12.9. This one has done well, scoring higher than 86% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 377,636 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 89% of its contemporaries.
We're also able to compare this research output to 9 others from the same source and published within six weeks on either side of this one. This one has scored higher than 6 of them.
