Epigenetic marks are likely to explain variability of response to antigen in granulomatous lung disease.
To identify DNA methylation and gene expression changes associated with chronic beryllium disease (CBD) and sarcoidosis in lung cells obtained by bronchoalveolar lavage (BAL).
BAL cells from CBD (n=8), BeS (n=8), sarcoidosis (n=8), and additional progressive (n=9) and remitting (n=15) cases of sarcoidosis were profiled on the Illumina 450K methylation and Affymetrix/Agilent gene expression microarrays. Statistical analyses were performed to identify DNA methylation and gene expression changes associated with CBD, sarcoidosis, and disease progression in sarcoidosis. DNA methylation array findings were validated by pyrosequencing.
We identified 52,860 significant (p<0.005 and q<0.05) CpGs associated with CBD; 2,726 CpGs near 1,944 unique genes have >25% methylation change. 69% of differentially methylated genes are significantly (q<0.05) differentially expressed in CBD, with many canonical inverse relationships of methylation and expression in genes critical to Th1 cell differentiation, chemokines and their receptors, and other genes involved in immunity. Testing of these CBD-associated CpGs in sarcoidosis reveals that methylation changes approach significance (q<0.15) and are methylated in the same direction, suggesting similarities between the two diseases with more heterogeneity in sarcoidosis. Analysis of progressive vs remitting sarcoidosis identified 15,215 CpGs (p<0.005 and q<0.05) but only 801 of them have >5% methylation change, demonstrating that DNA methylation marks of disease progression changes are more subtle.
Our study highlights the significance of epigenetic marks in lung immune response in granulomatous lung disease.
Pharmacology, Toxicology and Pharmaceutical Science
2
9%
Nursing and Health Professions
2
9%
Environmental Science
1
4%
Other
1
4%
Unknown
11
48%
Attention Score in Context
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 September 2023.
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#6,491,338
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Outputs from American Journal of Respiratory Cell and Molecular Biology
#676
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#105,988
of 487,042 outputs
Outputs of similar age from American Journal of Respiratory Cell and Molecular Biology
#10
of 53 outputs
Altmetric has tracked 33,704,742 research outputs across all sources so far. Compared to these this one has done well and is in the 79th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,229 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one has done well, scoring higher than 83% of its peers.
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We're also able to compare this research output to 53 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 81% of its contemporaries.
