A strategy for elucidating sequence determinants of function in the class of cytochrome P450 (CYP) enzymes that catalyze the first steps of terpene metabolism in wild microbiomes is described. Wild organisms that can use camphor, terpineol, pinene and limonene were isolated from soils rich in coniferous waste. Cell free extracts and growth beers were analyzed by gas chromatography/mass spectrometry to identify primary oxidative metabolites. For one organism, Pseudomonas nitroreducens TPJM, a cytochrome P450 (CYP108B1) isolated from cell free extracts was demonstrated to catalyze the oxidation of α-terpineol in assays combining the native ferredoxin and putidaredoxin reductase, and the resulting oxidation products identified by gas chromatography/mass spectrometry. Shotgun sequencing of PnTPJM identified four candidate P450 genes, including an apparently fragmentary gene with a high degree of homology with the known enzyme CYP108 (P450terp).
Pharmacology, Toxicology and Pharmaceutical Science
2
9%
Biochemistry, Genetics and Molecular Biology
2
9%
Nursing and Health Professions
2
9%
Psychology
1
5%
Other
1
5%
Unknown
9
41%
Attention Score in Context
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 September 2018.
All research outputs
#22,767,715
of 25,385,509 outputs
Outputs from Journal of Inorganic Biochemistry
#1,634
of 1,921 outputs
Outputs of similar age
#298,067
of 340,721 outputs
Outputs of similar age from Journal of Inorganic Biochemistry
#14
of 26 outputs
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