The purpose of this study was to identify how changes in retinal structure and function correlate with visual deficits during increasing amounts of retinal degeneration.
Retinal degeneration was induced in adult mice by subretinal injections of paraquat (PQ) (0.2-1 mM). Retinal anatomy and photoreceptor layer thickness were quantified by histology and optical coherence tomography (OCT), retinal function was measured using electroretinography (ERG), and visual behavior were measured by optokinetic tracking, at 1 to 3 week post-injury.
Photoreceptor layer structure, function and visual behavior declined at a linear rate over time following PQ-induced degeneration, with the correlations between outcome measures being lowest at mild injury levels and increasing with injury severity. Overall reductions in visual acuity were highly correlated with declines in retinal thickness (r(2) = 0.78) and function (r(2) = 0.67) and retinal thickness correlated with photoreceptor function (r(2) = 0.72). ERG a-wave scotopic amplitudes showed a stronger correspondence to retinal structure and visual behavior than b-waves.
Measurements of photoreceptor loss at the structural and functional levels showed good correspondence with degeneration-associated changes in visual behavior after oxidative stress injury. The results provide new insight about the relative kinetics of measurements of retinal degeneration induced by oxidative stress, which could guide the choice of optimal outcome measurements for other retinal diseases.
Pharmacology, Toxicology and Pharmaceutical Science
1
10%
Biochemistry, Genetics and Molecular Biology
1
10%
Neuroscience
1
10%
Unknown
5
50%
Attention Score in Context
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 November 2015.
All research outputs
#20,295,501
of 22,832,057 outputs
Outputs from Current Eye Research
#1,165
of 1,414 outputs
Outputs of similar age
#236,863
of 282,792 outputs
Outputs of similar age from Current Eye Research
#10
of 12 outputs
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