A sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for quantification of bictegravir in human plasma. A small volume of only 50 μL aliquot of plasma was precipitated with acetonitrile containing an internal standard (IS). Chromatographic separation was performed on Kinetex EVO C18 column, 50 × 3.0 mm, 5 μm using an isocratic mobile phase containing 80:20 acetonitrile/water with 0.1% formic acid. A mass spectrometer operated in ESI positive multiple reaction monitoring mode using the m/z 450.1/289.1 for bictegravir and 420.1/277.1 for IS. The dynamic range of the method was 1 to 10,000 ng/mL with a correlation coefficient (r) ≥ 0.9991. The precision results of calibration standards were in the range of 0.05 to 4.57% and accuracies were 95.07 to 104.70%. The mean extraction recovery was found to be 98.64% with a precision of 2.91%. The method was validated as per USFDA guidelines and was found to be accurate and precise. The method was successfully applied to in-vitro cellular uptake study.
Pharmacology, Toxicology and Pharmaceutical Science
7
25%
Medicine and Dentistry
3
11%
Chemistry
2
7%
Unknown
16
57%
Attention Score in Context
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 September 2018.
All research outputs
#21,885,607
of 24,417,958 outputs
Outputs from Biomedical Chromatography
#1,536
of 2,045 outputs
Outputs of similar age
#305,936
of 350,771 outputs
Outputs of similar age from Biomedical Chromatography
#16
of 50 outputs
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So far Altmetric has tracked 2,045 research outputs from this source. They receive a mean Attention Score of 2.1. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 50 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
