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@ArjunaRajakumar Thank you! It’s Scr (magenta), Ubx (cyan), abd-A (green), Abd-B (red). I skipped Antp b/c crustaceans form a weird Antp-Ubx hybrid transcript?, causing Antp probes to stain all thoracic appendages (see fig 6 from Serano et al 2016; 8C11 i…