Juan Maldonado, Jordan R. Yaron, Liqiang Zhang, Alexandra Lucas, Maldonado, Juan, Yaron, Jordan R., Zhang, Liqiang, Lucas, Alexandra
Abstract
Serine protease inhibitors, serpins, can have profound effects on many systems in the body including immune defense systems. The microbiome, specifically the gut and lung bacterial microbiota, is now known, under some conditions, to alter immune defenses. DNA library preparation for microbiome studies is a procedure that prepares microbial genomic DNA to be sequenced in next-generation sequencing platforms. The construction involves a PCR reaction that will amplify the 16S rRNA gene and will incorporate a specific index and adaptors to the fragments. After confirmation of the product amplification by gel electrophoresis, samples will be later normalized to the same DNA amount of 240 ng. Final concentration of the library is validated by quantitative PCR (qPCR).Here we describe methods to analyze changes in the microbiome after treatment with immune-modulating agents, specifically serpins.
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 July 2024.
All research outputs
#7,112,822
of 33,982,974 outputs
Outputs from Methods in molecular biology
#2,469
of 18,822 outputs
Outputs of similar age
#99,171
of 375,804 outputs
Outputs of similar age from Methods in molecular biology
#26
of 264 outputs
Altmetric has tracked 33,982,974 research outputs across all sources so far. Compared to these this one has done well and is in the 78th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 18,822 research outputs from this source. They receive a mean Attention Score of 4.1. This one has done well, scoring higher than 86% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 375,804 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.
We're also able to compare this research output to 264 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 90% of its contemporaries.
