Title |
Ultra‐performance liquid chromatography tandem mass spectrometry for the rapid, simultaneous analysis of ziprasidone and its impurities
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Published in |
Biomedical Chromatography, October 2018
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DOI | 10.1002/bmc.4384 |
Pubmed ID | |
Authors |
Marija Čarapić, Katarina Nikolic, Bojan Marković, Miloš Petković, Milena Pavlovic, Danica Agbaba |
Abstract |
Separation and characterization of the unknown degradation product of second generation antipsychotic drug ziprasidone was essential for defining genotoxic potential of the compound. The aim of this study was to develop simple UHPLC method coupled with tandem mass spectrometry (MS/MS) for chemical characterization of unknown degradant, separation and quantification of ziprasidone and its five main impurities (I-V) in raw material and pharmaceuticals. Chromatographic conditions were optimized by experimental design. The MS/MS fragmentation conditions were optimized individually for each compound in order to obtain both specific fragments and high signal intensity. A rapid and sensitive UHPLC-MS/MS method was developed. All seven analytes were eluted within the 7 min run time. The best separation was obtained on the Acquity UPLC BEH C18 (50 mm x 2.1 mm x 1.7 μm) column in gradient mode with ammonium-formate buffer (10mM; pH 4.7) and acetonitrile as mobile phase, with the flow rate of 0.3 mL min-1 and at the column temperature of 30o C. The new UHPLC-MS/MS method was fully validated and all validation parameters were confirmed. The fragmentation pathways and chemical characterization of unknown degradant was proposed and it was confirmed that there are no structural alert concerning genotoxicity. |
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Unknown | 1 | 100% |
Demographic breakdown
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Science communicators (journalists, bloggers, editors) | 1 | 100% |
Mendeley readers
Geographical breakdown
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Demographic breakdown
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Student > Master | 4 | 27% |
Other | 2 | 13% |
Lecturer | 1 | 7% |
Researcher | 1 | 7% |
Student > Postgraduate | 1 | 7% |
Other | 0 | 0% |
Unknown | 6 | 40% |
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Chemistry | 2 | 13% |
Agricultural and Biological Sciences | 1 | 7% |
Unknown | 7 | 47% |