| Title |
Harnessing the power of RADseq for ecological and evolutionary genomics
|
|---|---|
| Published in |
Nature Reviews Genetics, January 2016
|
| DOI | 10.1038/nrg.2015.28 |
| Pubmed ID | |
| Authors | |
| Abstract |
High-throughput techniques based on restriction site-associated DNA sequencing (RADseq) are enabling the low-cost discovery and genotyping of thousands of genetic markers for any species, including non-model organisms, which is revolutionizing ecological, evolutionary and conservation genetics. Technical differences among these methods lead to important considerations for all steps of genomics studies, from the specific scientific questions that can be addressed, and the costs of library preparation and sequencing, to the types of bias and error inherent in the resulting data. In this Review, we provide a comprehensive discussion of RADseq methods to aid researchers in choosing among the many different approaches and avoiding erroneous scientific conclusions from RADseq data, a problem that has plagued other genetic marker types in the past. |
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X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 7 | 16% |
| United Kingdom | 7 | 16% |
| Germany | 2 | 5% |
| Canada | 2 | 5% |
| Brazil | 2 | 5% |
| Switzerland | 2 | 5% |
| Iraq | 1 | 2% |
| Denmark | 1 | 2% |
| Thailand | 1 | 2% |
| Other | 5 | 12% |
| Unknown | 13 | 30% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 22 | 51% |
| Scientists | 20 | 47% |
| Science communicators (journalists, bloggers, editors) | 1 | 2% |
Mendeley demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 23 | <1% |
| Italy | 4 | <1% |
| France | 3 | <1% |
| Brazil | 3 | <1% |
| Portugal | 3 | <1% |
| United Kingdom | 3 | <1% |
| Netherlands | 3 | <1% |
| Germany | 3 | <1% |
| Canada | 2 | <1% |
| Other | 23 | <1% |
| Unknown | 2990 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 697 | 23% |
| Student > Master | 505 | 17% |
| Researcher | 492 | 16% |
| Student > Bachelor | 284 | 9% |
| Student > Doctoral Student | 168 | 5% |
| Other | 345 | 11% |
| Unknown | 569 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
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| Biochemistry, Genetics and Molecular Biology | 542 | 18% |
| Environmental Science | 211 | 7% |
| Earth and Planetary Sciences | 21 | <1% |
| Engineering | 15 | <1% |
| Other | 99 | 3% |
| Unknown | 664 | 22% |