@DanTGrimes I know there’s some concern about p53-mediated fell cycle arrest induced by CRISPR cutting, so that’s an argument for using an orthogonal knockdown technique (though morpholinos have their own p53 considerations). https://t.co/Z9WvP90C1N http…
RT @jonmoulton: Lai et al. 2018. https://t.co/EtwDjh3z1l Robu et al. 2007. https://t.co/YRxdXXSjlM
Lai et al. 2018. https://t.co/EtwDjh3z1l Robu et al. 2007. https://t.co/YRxdXXSjlM
@DocDellaire @GaetanBurgio @doyonlab @jcornlab p53 is activated by all sorts of genome engineering tools including siRNA, PNAs and MOs. Nothing noted for ZFNs or TALENs AFAICT. Makes one wonder whether the issue are the gRNAs rather than the DSBs. https…