Acute pancreatitis (AP) is a common abdominal inflammatory disease. Disturbed gut homeostasis secondary to pancreatic inflammation aggravates the condition retroactively. Probiotic Clostridium butyricum (C. butyricum) strains with immunomodulatory effects are known to suppress intestinal inflammation. The current study investigated potential beneficial effects of C. butyricum strains on AP and underlying mechanisms.
Experimental AP or severe AP (SAP) was induced by caerulein hyperstimulation or caerulein hyperstimulation together with lipopolysaccharide. Two C. butyricum strains MIYAIRI 588 (CBM588) and CGMCC0313.1 (CB0313.1) were supplemented to mice for three weeks before AP or SAP induction. We found that both CBM588 and CB0313.1 protected against experimental AP, as evidenced by reduced serum amylase and lipase levels, pancreatic edema and myeloperoxidase activity. Amelioration of both experimental AP and SAP by CB0313.1 indicated a non-model specific effect. Moreover, C. butyricum inhibited pancreatic neutrophil and dendritic cell infiltration, NLRP3 inflammasome activation as well as pro-inflammatory signaling pathways. Additionally in the gut, C. butyricum strains attenuated AP-associated intestinal inflammation and barrier dysfunction. Reduced penetration of pathogenic bacteria Escherichia coli and Enterococcus in pancreas was observed as a result of reinforced gut barrier function. Gut microbiome analyses further revealed that beneficial effects of C. butyricum on pancreatic-gut homeostasis were correlated with improved gut dysbiosis. In particular, relative abundance of Desulfovibrionaceae decreased, and Verrucomicrobiaceae Clostridiaceae and Lactobacillaceae increased.
We for the first time demonstrate a protective effect of C. butyricum in AP by modulating intestinal homeostasis. This study identifies feasible probiotic-candidates for AP treatment in clinical settings. This article is protected by copyright. All rights reserved.
Pharmacology, Toxicology and Pharmaceutical Science
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4%
Medicine and Dentistry
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4%
Other
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Unknown
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48%
Attention Score in Context
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 September 2022.
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#4,242,110
of 28,695,121 outputs
Outputs from Molecular Nutrition & Food Research
#750
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Outputs of similar age
#75,943
of 368,954 outputs
Outputs of similar age from Molecular Nutrition & Food Research
#12
of 46 outputs
Altmetric has tracked 28,695,121 research outputs across all sources so far. Compared to these this one has done well and is in the 85th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,876 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 14.3. This one has gotten more attention than average, scoring higher than 73% of its peers.
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We're also able to compare this research output to 46 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.
