To characterise growth factor release from dentine by pulp capping agents and to determine the effects of liberated dentine extracellular matrix (dECM) components on pulp cells in the key wound healing processes of migration and cell growth.
Powdered human dentine was exposed to solutions of calcium hydroxide, white and grey Mineral Trioxide Aggregate (MTA) (ProRoot, (Dentsply Tulsa, Tulsa, OK, USA) over 14 days. The solubilised dECM components were dialysed and lyophilised and characterised using multiplex quantitative ELISA. Following dECM component extraction dentine was analysed using Fourier transform infrared spectroscopy (FTIR). Primary rat dental pulp cells (RDPCs) were exposed to dECM components (0.1 - 100 μg/mL) released by calcium hydroxide, white and grey MTA and cell growth and chemotactic responses were assessed. Statistical differences between the experimental and control groups were determined using one way ANOVA RESULTS: A broad range of growth factors, many not previously reported in dentine, were liberated by these pulp capping agents, including: SCF, M-CSF, GM-CSF, IGFBP-1, NGF, GDNF. White and grey MTA liberated more growth factors than calcium hydroxide. FTIR analysis of dentine exposed to pulp capping agents showed partial depletion of amide bands I, II and III, with little alteration in phosphate peaks compared to untreated dentine. dECM components released by white and grey MTA induced significantly more cell growth at low-to-moderate concentrations (p≤0.05) examined in this study, and significantly enhanced cell chemotaxis at all concentrations compared with controls (p≤0.05).
White and grey MTA solubilise a broad range of bioactive molecules from dentine, which can induce proliferation and chemotaxis in pulp cells. This article is protected by copyright. All rights reserved.
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 March 2016.
All research outputs
#19,290,136
of 24,558,777 outputs
Outputs from International Endodontic Journal
#678
of 1,072 outputs
Outputs of similar age
#210,614
of 305,015 outputs
Outputs of similar age from International Endodontic Journal
#8
of 39 outputs
Altmetric has tracked 24,558,777 research outputs across all sources so far. This one is in the 18th percentile – i.e., 18% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,072 research outputs from this source. They receive a mean Attention Score of 2.9. This one is in the 33rd percentile – i.e., 33% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 305,015 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 26th percentile – i.e., 26% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 39 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 74% of its contemporaries.
