To assess the biological effects of passage through clinically relevant needles on the viability and metabolic activity of culture-expanded, human adipose derived mesenchymal stromal/stem cells (AMSCs).
Prospective observational pilot study.
Academic medical center.
Patient-derived clinical-grade culture expanded AMSCs.
AMSCs were passed through syringes without a needle attached (control), with an 18-gauge (25.4mm) needle attached and with a 30-gauge (19mm) needle attached at a constant injection flow rate and constant cell concentrations. Each injection condition was completed in triplicate.
Cell number and viability, proliferative capacity, metabolic activity, and acute gene expression as measured by cell counts, mitochondrial activity, and quantitative real time reverse-transcriptase polymerase chain reaction (qRT-PCR) on day 0 (immediately), day 1, and day 4 after injection.
AMSC viability was not significantly affected by injection and cells proliferated normally regardless of study group. Post-injection, AMSCs robustly expressed both proliferation markers and extracellular matrix proteins. Stress-response mRNAs were markedly but transiently increased independently of needle size within the first day in culture post-injection.
Human, culture expanded AMSCs maintain their viability, proliferative capacity and metabolic function following passage through needles as small as 30 gauge at constant flow rates of 4 ml/min, despite an early, non-specific stress/cytoprotective response. These initial findings suggest that culture expanded AMSCs should tolerate the injection process during most cell-based therapeutic interventions.
This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 February 2016.
All research outputs
#7,662,775
of 28,833,811 outputs
Outputs from PM&R
#616
of 1,876 outputs
Outputs of similar age
#104,045
of 416,958 outputs
Outputs of similar age from PM&R
#8
of 49 outputs
Altmetric has tracked 28,833,811 research outputs across all sources so far. This one has received more attention than most of these and is in the 72nd percentile.
So far Altmetric has tracked 1,876 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 11.6. This one has gotten more attention than average, scoring higher than 66% of its peers.
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We're also able to compare this research output to 49 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 83% of its contemporaries.
