Indeed, we previously showed that Aca2 autorepresses this operon. Repression occured via two conserved inverted repeats (IRs) upstream of acrIF8—but only one them was bound by Aca2 in DNA shift assays. Mysterious! 🕵🏻♂️ https://t.co/wrJyUJcA6H
Because this strain could only take up DNA with RM-compatible methylation, we knocked out the restriction endonuclease of the RM system, enabling us to pursue our anti-CRISPR regulation project: https://t.co/8fbtslwoir And that could have been the end…
RT @NilsBirkholz: Happy to see our new study on anti-CRISPR regulation by an Aca protein published by @NAR_Open, thanks to great effort by…
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