Katherine L Doll, Lecia L Pewe, Samarchith P Kurup, John T Harty

Despite decades of research, malaria remains a global health crisis. Current subunit vaccine approaches do not provide efficient long-term, sterilizing immunity againstPlasmodiuminfections in humans. Conversely, whole parasite vaccinations with their larger array of target Ags have conferred long-lasting sterilizing protection to humans. Similar studies in rodent models of malaria reveal that CD8(+)T cells play a critical role in liver-stage immunity after whole parasite vaccination. However, it is unknown whether all CD8(+)T cell specificities elicited by whole parasite vaccination contribute to protection, an issue of great relevance for enhanced subunit vaccination. In this article, we show that robust CD8(+)T cell responses of similar phenotype are mounted after prime-boost immunization againstPlasmodium bergheiglideosome-associated protein 5041-48-, sporozoite-specific protein 20318-325-,thrombospondin-related adhesion protein (TRAP) 130-138-, or circumsporozoite protein (CSP) 252-260-derived epitopes in mice, but only CSP252-260- and TRAP130-138-specific CD8(+)T cells provide sterilizing immunity and reduce liver parasite burden after sporozoite challenge. Further, CD8(+)T cells specific to sporozoite surface-expressed CSP and TRAP proteins, but not intracellular glideosome-associated protein 50 and sporozoite-specific protein 20, efficiently recognize sporozoite-infected hepatocytes in vitro. These results suggest that: 1) protection-relevant antigenic targets, regardless of their immunogenic potential, must be efficiently presented by infected hepatocytes for CD8(+)T cells to eliminate liver-stagePlasmodiuminfection; and 2) proteins expressed on the surface of sporozoites may be good target Ags for protective CD8(+)T cells.