The spread of virus-induced infectious diseases through airborne routes of transmission is a global concern for economic and medical reasons. To study virus transmission, it is essential to have an effective aerosol collector such as the growth tube collector (GTC) system that utilizes water-based condensation for collecting virus-containing aerosols. In this work we characterized the GTC system using bacteriophage MS2 as a surrogate for a small RNA virus. We investigated using RNA extraction and reverse-transcriptase polymerase chain reaction (RT-PCR) to study the total virus collection efficiency of the GTC system. We also used a plaque assay (culturing collected virus samples) to characterize the GTC system and compared GTC with a commercially available apparatus SKC(®) Biosampler. The plaque assay reports only viable viruses collected whereas RT-PCR analyzes total viruses, including those viruses inactivated during collection. The effects of relative humidity (RH) and other conditions on collection efficiency were also investigated. Our results suggest that GTC has collection efficiency of viable viruses between 0.24 - 1.8% and total collection efficiency between 18.3 - 79.0%, which is 1 - 2 orders of magnitude higher than SKC(®) Biosampler. Moreover, higher RH significantly increases both of the viable and total collection efficiency of GTC while its effect on the collection efficiency of SKC(®) Biosampler is not significant. This article is protected by copyright. All rights reserved.
This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 December 2019.
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#10,119,438
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#9
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Altmetric has tracked 34,364,397 research outputs across all sources so far. This one has received more attention than most of these and is in the 70th percentile.
So far Altmetric has tracked 5,103 research outputs from this source. They receive a mean Attention Score of 4.4. This one has gotten more attention than average, scoring higher than 68% of its peers.
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We're also able to compare this research output to 28 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 67% of its contemporaries.
