An in vivo strategy for knockdown of circular RNAs

But what about functions in trans? To tackle this question we needed a genetic tool to manipulate levels of circRNA without affecting linear counterpart. For this, the lab developed a tool using shRNAs https://t.co/9VNlfCKgkT 9/

#circRNAs contribute to gene regulation by sponging #microRNAs, interacting with RNA-binding proteins, regulating #transcription and #splicing, and protein translation. They play critical roles in the nervous system. This paper is about downregulating s…