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Isoniazid and rifampin drug susceptibility testing: application of 2,3,5-triphenyl tetrazolium chloride assay and microscopic-observation drug-susceptibility assay directly on Ziehl-Neelsen smear…

Overview of attention for article published in Brazilian Journal of Infectious Diseases, November 2015
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Title
Isoniazid and rifampin drug susceptibility testing: application of 2,3,5-triphenyl tetrazolium chloride assay and microscopic-observation drug-susceptibility assay directly on Ziehl-Neelsen smear positive sputum specimens
Published in
Brazilian Journal of Infectious Diseases, November 2015
DOI 10.1016/j.bjid.2015.09.006
Pubmed ID
Authors

Pottathil Shinu, Varsha Singh, Anroop Nair

Abstract

The current study was aimed to evaluate the performance of direct 2,3,5-triphenyl tetrazolium chloride assay and direct microscopic observation drug susceptibility assay with indirect Löwenstein-Jensen proportion method directly on Ziehl-Neelsen smear positive sputum specimens. Direct acid fast bacilli smear positive sputum specimens (n=264) were subjected to isoniazid and rifampicin drug susceptibility testing by direct 2,3,5-triphenyl tetrazolium chloride assay, direct microscopic observation drug susceptibility assay, and the performances were compared with indirect Löwenstein-Jensen proportion method. The direct 2,3,5-triphenyl tetrazolium chloride assay demonstrated an overall sensitivity, specificity, positive predictive value, and negative predictive value of 99.2%, 82.4%, 99.2%, and 88.5%, respectively, for the detection of isoniazid and rifampicin resistant Mycobacterium tuberculosis isolates when compared to indirect Löwenstein-Jensen proportion method. Likewise, the overall sensitivity, specificity, positive predictive value and negative predictive value of direct microscopic observation drug susceptibility assay were 98.8%, 82.4%, 99.2%, and 78.2%, respectively. The direct 2,3,5-triphenyl tetrazolium chloride assay was found to be an economical alternative method for the rapid and accurate detection of isoniazid and rifampicin resistance from direct acid fast bacilli smear positive sputum specimens.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Portugal 1 3%
Canada 1 3%
Unknown 27 93%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 21%
Student > Ph. D. Student 4 14%
Student > Bachelor 3 10%
Student > Doctoral Student 2 7%
Student > Master 2 7%
Other 4 14%
Unknown 8 28%
Readers by discipline Count As %
Medicine and Dentistry 8 28%
Pharmacology, Toxicology and Pharmaceutical Science 5 17%
Immunology and Microbiology 3 10%
Social Sciences 2 7%
Veterinary Science and Veterinary Medicine 1 3%
Other 1 3%
Unknown 9 31%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 August 2016.
All research outputs
#23,208,433
of 25,864,668 outputs
Outputs from Brazilian Journal of Infectious Diseases
#648
of 815 outputs
Outputs of similar age
#338,982
of 396,516 outputs
Outputs of similar age from Brazilian Journal of Infectious Diseases
#13
of 20 outputs
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