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Expression of cry1Ab gene from a novel Bacillus thuringiensis strain SY49-1 active on pest insects

Overview of attention for article published in Brazilian Journal of Microbiology, April 2016
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Title
Expression of cry1Ab gene from a novel Bacillus thuringiensis strain SY49-1 active on pest insects
Published in
Brazilian Journal of Microbiology, April 2016
DOI 10.1016/j.bjm.2016.04.011
Pubmed ID
Authors

Ugur Azizoglu, Abdurrahman Ayvaz, Semih Yılmaz, Salih Karabörklü, Rıdvan Temizgul

Abstract

In this study, the cry1Ab gene of previously characterized and Lepidoptera-, Diptera-, and Coleoptera-active Bacillus thuringiensis SY49-1 strain was cloned, expressed and individually tested on Ephestia kuehniella (Lepidoptera: Pyralidae) and Plodia interpunctella (Lepidoptera: Pyralidae) larvae. pET-cry1Ab plasmids were constructed by ligating the cry1Ab into pET28a (+) expression vector. Constructed plasmids were transferred to an Escherichia coli BL21 (DE3) strain rendered competent with CaCl2. Isopropyl β-d-1-thiogalactopyranoside was used to induce the expression of cry1Ab in E. coli BL21(DE3), and consequently, ∼130kDa of Cry1Ab was obtained. Bioassay results indicated that recombinant Cry1Ab at a dose of 1000μgg(-1) caused 40% and 64% mortality on P. interpunctella and E. kuehniella larvae, respectively. However, the mortality rates of Bt SY49-1 strains' spore-crystal mixture at the same dose were observed to be 70% on P. interpunctella and 90% on E. kuehniella larvae. The results indicated that cry1Ab may be considered as a good candidate in transgenic crop production and as an alternative biocontrol agent in controlling stored product moths.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 41 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 41 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 17%
Researcher 5 12%
Student > Doctoral Student 4 10%
Student > Bachelor 3 7%
Student > Postgraduate 3 7%
Other 6 15%
Unknown 13 32%
Readers by discipline Count As %
Agricultural and Biological Sciences 16 39%
Biochemistry, Genetics and Molecular Biology 7 17%
Unspecified 1 2%
Immunology and Microbiology 1 2%
Social Sciences 1 2%
Other 1 2%
Unknown 14 34%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 May 2016.
All research outputs
#22,758,309
of 25,373,627 outputs
Outputs from Brazilian Journal of Microbiology
#1,047
of 1,377 outputs
Outputs of similar age
#270,480
of 313,434 outputs
Outputs of similar age from Brazilian Journal of Microbiology
#19
of 20 outputs
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