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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Production of recombinant human epidermal growth factor in Pichia pastoris
|
|---|---|
| Published in |
Brazilian Journal of Microbiology, April 2017
|
| DOI | 10.1016/j.bjm.2016.10.017 |
| Pubmed ID | |
| Authors |
Samira Eissazadeh, Hassan Moeini, Maryam Ghandizadeh Dezfouli, Somayyeh Heidary, Rubina Nelofer, Mohd Puad Abdullah |
| Abstract |
This study was carried out to express human epidermal growth factor (hEGF) in Pichia pastoris GS115. For this aim, the hEGF gene was cloned into the pPIC9K expression vector, and then integrated into P. pastoris by electroporation. ELISA-based assay showed that the amount of hEGF secreted into the medium can be affected by the fermentation conditions especially by culture medium, pH and temperature. The best medium for the optimal hEGF production was BMMY buffered at a pH range of 6.0 and 7.0. The highest amount of hEGF with an average yield of 2.27μg/mL was obtained through an induction of the culture with 0.5% (v/v) methanol for 60h. The artificial neural network (ANN) analysis revealed that changes in both pH and temperature significantly affected the hEGF production with the pH change had slightly higher impact on hEGF production than variations in the temperature. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Colombia | 1 | 50% |
| France | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 124 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 124 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 28 | 23% |
| Student > Master | 16 | 13% |
| Researcher | 12 | 10% |
| Student > Ph. D. Student | 11 | 9% |
| Other | 4 | 3% |
| Other | 7 | 6% |
| Unknown | 46 | 37% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 39 | 31% |
| Agricultural and Biological Sciences | 11 | 9% |
| Chemistry | 7 | 6% |
| Engineering | 5 | 4% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 2% |
| Other | 10 | 8% |
| Unknown | 49 | 40% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 April 2017.
All research outputs
#19,947,956
of 25,382,440 outputs
Outputs from Brazilian Journal of Microbiology
#824
of 1,377 outputs
Outputs of similar age
#235,251
of 324,698 outputs
Outputs of similar age from Brazilian Journal of Microbiology
#4
of 8 outputs
Altmetric has tracked 25,382,440 research outputs across all sources so far. This one is in the 18th percentile – i.e., 18% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,377 research outputs from this source. They receive a mean Attention Score of 3.6. This one is in the 35th percentile – i.e., 35% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 324,698 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 23rd percentile – i.e., 23% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 8 others from the same source and published within six weeks on either side of this one. This one has scored higher than 4 of them.