You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
X Demographics
Mendeley readers
Attention Score in Context
| Title |
A two-plasmid strategy for engineering a dengue virus type 3 infectious clone from primary Brazilian isolate
|
|---|---|
| Published in |
Anais da Academia Brasileira de Ciências, December 2014
|
| DOI | 10.1590/0001-3765201420130332 |
| Pubmed ID | |
| Authors |
JEFFERSON J.S. SANTOS, MARLI T. CORDEIRO, GIOVANI R. BERTANI, ERNESTO T.A. MARQUES, LAURA H.V.G. GIL |
| Abstract |
Dengue infections represent one of the most prevalent arthropod-borne diseases worldwide, causing a wide spectrum of clinical outcomes. Engineered infectious clone is an important tool to study Dengue virus (DENV) biology. Functional full-length cDNA clones have been constructed for many positive-strand RNA viruses and have provided valuable tools for studying the molecular mechanisms involved in viral genome replication, virion assembly, virus pathogenesis and vaccine development. We report herein the successful development of an infectious clone from a primary Brazilian isolate of dengue virus 3 (DENV3) of the genotype III. Using a two-plasmid strategy, DENV3 genome was divided in two parts and cloned separately into a yeast-bacteria shuttle vector. All plasmids were assembled in yeast by homologous recombination technique and a full-length template for transcription was obtained by in vitro ligation of the two parts of the genome. Transcript-derived DENV3 is infectious upon transfection into BHK-21 cells and in vitro characterization confirmed its identity. Growth kinetics of transcript-derived DENV3 was indistinguishable from wild type DENV3. This system is a powerful tool that will help shed light on molecular features of DENV biology, as the relationship of specific mutations and DENV pathogenesis. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 1 | 4% |
| Unknown | 25 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 6 | 23% |
| Student > Ph. D. Student | 4 | 15% |
| Student > Doctoral Student | 3 | 12% |
| Student > Postgraduate | 3 | 12% |
| Other | 2 | 8% |
| Other | 7 | 27% |
| Unknown | 1 | 4% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 8 | 31% |
| Medicine and Dentistry | 5 | 19% |
| Agricultural and Biological Sciences | 5 | 19% |
| Immunology and Microbiology | 3 | 12% |
| Veterinary Science and Veterinary Medicine | 1 | 4% |
| Other | 3 | 12% |
| Unknown | 1 | 4% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 January 2015.
All research outputs
#23,320,957
of 25,988,468 outputs
Outputs from Anais da Academia Brasileira de Ciências
#23
of 217 outputs
Outputs of similar age
#318,553
of 372,165 outputs
Outputs of similar age from Anais da Academia Brasileira de Ciências
#9
of 12 outputs
Altmetric has tracked 25,988,468 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 217 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.1. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 372,165 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 12 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.