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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Pimenta pseudocaryophyllus inhibits virulence factors and promotes metabolic changes in Candidayeast
|
|---|---|
| Published in |
Revista da Sociedade Brasileira de Medicina Tropical, October 2014
|
| DOI | 10.1590/0037-8682-0135-2014 |
| Pubmed ID | |
| Authors |
Flávio Ezeddinne El Assal, Joelma Abadia Marciano Paula, Leonardo Silva Capeletti, Fernando Yano Abrão, Fábio Silvestre Ataídes, Fabyola Amaral da Silva Sá, Carolina Rodrigues Costa, Orionalda de Fátima Lisboa Fernandes, Lúcia Kioko Hasimoto Souza, Maria do Rosario Rodrigues Silva |
| Abstract |
Introduction This is the first study to examine the in vitro susceptibility and the expression of virulence factors in Candida species in the presence of Pimenta pseudocaryophyllus (Gomes) L.R. Landrum (Myrtaceae), a Brazilian plant known as paucravo. Additionally, the mechanisms of action of the crude ethanol extract and the ethyl acetate and aqueous fractions of this plant were investigated. Methods The in vitro susceptibility of Candida was tested using the broth microdilution method, whereas an XTT reduction assay was used for biofilms. Adherence was determined by counting the number of yeast cells that adhered to 100 oral epithelial cells, and hyphal formation was verified in the hyphal induction medium M199. Flow cytometry with propidium iodide and FUN-1 was performed to assess the mechanism of action. Results The results revealed that the crude ethanol extract and the ethyl acetate and aqueous fractions of P. pseudocaryophyllus inhibited the growth of Candida isolates at a minimal inhibitory concentration (MIC) ranging from 64 to 256µg/mL, whereas the 50% sessile minimal inhibitory concentration (SMIC50) ranged from 512 to >1,024µg/mL. Adherence and hyphal formation were significantly reduced in the presence of the crude ethanol extract and both fractions. Although cell membrane injury was detected, the predominant mechanism of action appeared to be the alteration of yeast metabolism, as demonstrated by flow cytometry. Conclusions Our results indicated that antifungal activity reduced the expression of virulence factors in yeast via the alteration of yeast metabolism, suggesting that the crude extract of P. pseudocaryophyllus and its fractions may contain novel antifungal agents. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 28 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 7 | 25% |
| Student > Bachelor | 4 | 14% |
| Student > Doctoral Student | 3 | 11% |
| Student > Postgraduate | 3 | 11% |
| Student > Ph. D. Student | 2 | 7% |
| Other | 4 | 14% |
| Unknown | 5 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 5 | 18% |
| Immunology and Microbiology | 3 | 11% |
| Biochemistry, Genetics and Molecular Biology | 3 | 11% |
| Nursing and Health Professions | 2 | 7% |
| Medicine and Dentistry | 2 | 7% |
| Other | 7 | 25% |
| Unknown | 6 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 December 2014.
All research outputs
#22,756,649
of 25,371,288 outputs
Outputs from Revista da Sociedade Brasileira de Medicina Tropical
#953
of 1,193 outputs
Outputs of similar age
#227,182
of 265,618 outputs
Outputs of similar age from Revista da Sociedade Brasileira de Medicina Tropical
#12
of 13 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,193 research outputs from this source. They receive a mean Attention Score of 2.9. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 265,618 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 13 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.