Title |
Molecular diagnosis of cutaneous leishmaniasis in an endemic area of Acre State in the Amazonian Region of Brazil
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Published in |
Revista da Sociedade Brasileira de Medicina Tropical, June 2018
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DOI | 10.1590/0037-8682-0232-2017 |
Pubmed ID | |
Authors |
Thais de Araujo-Pereira, Daniela de Pita-Pereira, Regina Barbosa Moreira, Tainah Silva-Galdino, Márcia P. de Oliveira Duarte, Reginaldo Peçanha Brazil, Constança Britto |
Abstract |
This study proposes to identify the Leishmania species found in the skin lesions of cutaneous leishmaniasis (CL) patients from Brasiléia municipality (Acre). Skin biopsy imprints or biopsy fragments were assayed via kDNA-PCR/RFLP and FRET-real-time PCR. Of individuals with suspected CL, 18 were positive for Leishmania kDNA. Leishmania (Viannia) braziliensis (61.1%) and Leishmania (Viannia) guyanensis (5.5%) were identified in the positive samples. These results are congruent with the previous reports in Acre and Bolivia, revealing L. braziliensis as the most prevalent species. L. guyanensis identification also corroborates with the epidemiology of the disease in the Amazon Basin. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 50 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 9 | 18% |
Student > Bachelor | 6 | 12% |
Researcher | 5 | 10% |
Other | 3 | 6% |
Student > Ph. D. Student | 2 | 4% |
Other | 4 | 8% |
Unknown | 21 | 42% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 6 | 12% |
Medicine and Dentistry | 5 | 10% |
Pharmacology, Toxicology and Pharmaceutical Science | 4 | 8% |
Agricultural and Biological Sciences | 4 | 8% |
Nursing and Health Professions | 2 | 4% |
Other | 6 | 12% |
Unknown | 23 | 46% |