Title |
Microsatellite markers: what they mean and why they are so useful
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Published in |
Genetics and Molecular Biology, August 2016
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DOI | 10.1590/1678-4685-gmb-2016-0027 |
Pubmed ID | |
Authors |
Maria Lucia Carneiro Vieira, Luciane Santini, Augusto Lima Diniz, Carla de Freitas Munhoz |
Abstract |
Microsatellites or Single Sequence Repeats (SSRs) are extensively employed in plant genetics studies, using both low and high throughput genotyping approaches. Motivated by the importance of these sequences over the last decades this review aims to address some theoretical aspects of SSRs, including definition, characterization and biological function. The methodologies for the development of SSR loci, genotyping and their applications as molecular markers are also reviewed. Finally, two data surveys are presented. The first was conducted using the main database of Web of Science, prospecting for articles published over the period from 2010 to 2015, resulting in approximately 930 records. The second survey was focused on papers that aimed at SSR marker development, published in the American Journal of Botany's Primer Notes and Protocols in Plant Sciences (over 2013 up to 2015), resulting in a total of 87 publications. This scenario confirms the current relevance of SSRs and indicates their continuous utilization in plant science. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Brazil | 3 | <1% |
Pakistan | 1 | <1% |
Unknown | 1618 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 283 | 17% |
Student > Master | 263 | 16% |
Student > Ph. D. Student | 255 | 16% |
Researcher | 155 | 10% |
Student > Doctoral Student | 71 | 4% |
Other | 175 | 11% |
Unknown | 420 | 26% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 575 | 35% |
Biochemistry, Genetics and Molecular Biology | 385 | 24% |
Environmental Science | 63 | 4% |
Immunology and Microbiology | 24 | 1% |
Medicine and Dentistry | 18 | 1% |
Other | 101 | 6% |
Unknown | 456 | 28% |