Title |
DNA sampling from eggshells and microsatellite genotyping in rare tropical birds: Case study on Brazilian Merganser
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Published in |
Genetics and Molecular Biology, October 2017
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DOI | 10.1590/1678-4685-gmb-2016-0297 |
Pubmed ID | |
Authors |
Thais Augusta Maia, Sibelle Torres Vilaça, Luciana Resende da Silva, Fabricio Rodrigues Santos, Gisele Pires de Mendonça Dantas |
Abstract |
This study shows that sampling maternal DNA from hatched and abandoned eggshells is a viable noninvasive strategy for studying the genetics of rare or endangered tropical birds, as exemplified here by the Brazilian Merganser (Mergus octosetaceus). Eighteen microsatellites were isolated from enriched libraries and nine heterologous loci from related species were tested. Seven loci were amplified successfully, with five of them being polymorphic. These loci exhibited amplicons ranging from 110 to 254 bp for 132 samples, with 60 from eggshells and 72 from blood or muscle samples. The number of alleles for M. octosetaceus ranged from one to six (mean = 3.71), which is low compared to M. merganser (1-15 alleles), a 'least concern' species. Genetic diversity did not differ significantly between noninvasive and invasive samples (Z(u) = 0.31, p = 0.37). Thus, noninvasive sampling, as demonstrated here with eggshells, provides an efficient means to assess genetic diversity in tropical birds without the need to capture and handle them. |
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Geographical breakdown
Country | Count | As % |
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United Kingdom | 2 | 40% |
Brazil | 1 | 20% |
Germany | 1 | 20% |
Unknown | 1 | 20% |
Demographic breakdown
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Members of the public | 4 | 80% |
Scientists | 1 | 20% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 24 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 6 | 25% |
Student > Bachelor | 3 | 13% |
Researcher | 3 | 13% |
Student > Ph. D. Student | 3 | 13% |
Other | 1 | 4% |
Other | 0 | 0% |
Unknown | 8 | 33% |
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Agricultural and Biological Sciences | 10 | 42% |
Biochemistry, Genetics and Molecular Biology | 3 | 13% |
Environmental Science | 1 | 4% |
Nursing and Health Professions | 1 | 4% |
Veterinary Science and Veterinary Medicine | 1 | 4% |
Other | 0 | 0% |
Unknown | 8 | 33% |