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Attention Score in Context
| Title |
Two fast screening methods (GC-MS and TLC-ChEI assay) for rapid evaluation of potential anticholinesterasic indole alkaloids in complex mixtures
|
|---|---|
| Published in |
Anais da Academia Brasileira de Ciências, September 2008
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| DOI | 10.1590/s0001-37652008000300003 |
| Pubmed ID | |
| Authors |
Ivo J.C. Vieira, Walter L.B. Medeiros, Cecilia S. Monnerat, Jucimar J. Souza, Leda Mathias, Raimundo Braz-Filho, Angelo C. Pinto, Priscila M. Sousa, Claudia M. Rezende, Rosângela De A. Epifanio |
| Abstract |
The pharmacotherapy for Alzheimer's disease (AD) includes the use of acetylcholinesterase inhibitors (AChEI). Recent investigations for novel AD therapeutic agents from plants suggested that Tabernaemontana genus is a promising source of novel anticholinesterasic indole alkaloids. In this work two fast screening techniques were combined in order to easily identify novel cholinesterase inhibitors (ChEI). Gas chromatography-mass spectrometry (GC-MS) of the less polar alkaloidic fractions obtained from the acid-base extraction of the stalk of T. laeta revealed thirteen monoindole alkaloids, four of them confirmed by co-injection with previously isolated alkaloids. The others were tentatively identified by mass fragmentation analysis. By gas chromatography with flame ionization detection (GC-FID) and using isatin as internal standard, affinisine and voachalotine were determined as major compounds. These fractions and fourteen previously isolated alkaloids, obtained from root bark of T. laeta and T. hystrix were investigated for acetyl (AChE) and butyrylcholinesterase (BuChE) inhibitory activities by the modified Ellman's method in thin layer chromatography(TLC-ChEI). Results showed selective inhibition of the alkaloids heyneanine and Nb-methylvoachalotine for BuChE, and 19-epi-isovoacristine for AChE, whereas olivacine, affinisine, ibogamine, affinine, conodurine and hystrixnine inhibited both enzymes. In addition to confirming that monoterpenoid indole alkaloids can be novel therapeutic agents for AD, this is the first report of the ChEI activity of olivacine, a pyridocarbazole alkaloid. |
Mendeley readers
The data shown below were compiled from readership statistics for 50 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Algeria | 1 | 2% |
| Brazil | 1 | 2% |
| Unknown | 48 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 7 | 14% |
| Researcher | 6 | 12% |
| Student > Bachelor | 5 | 10% |
| Student > Ph. D. Student | 5 | 10% |
| Student > Doctoral Student | 4 | 8% |
| Other | 13 | 26% |
| Unknown | 10 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 11 | 22% |
| Agricultural and Biological Sciences | 7 | 14% |
| Biochemistry, Genetics and Molecular Biology | 3 | 6% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 6% |
| Neuroscience | 2 | 4% |
| Other | 7 | 14% |
| Unknown | 17 | 34% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 May 2022.
All research outputs
#8,783,469
of 25,986,827 outputs
Outputs from Anais da Academia Brasileira de Ciências
#1
of 3 outputs
Outputs of similar age
#35,769
of 96,933 outputs
Outputs of similar age from Anais da Academia Brasileira de Ciências
#1
of 2 outputs
Altmetric has tracked 25,986,827 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3 research outputs from this source. They receive a mean Attention Score of 2.2. This one scored the same or higher as 2 of them.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 96,933 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 2 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them