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Mendeley readers
| Title |
Non-invasive genetic sampling for molecular sexing and microsatellite genotyping of hyacinth macaw (Anodorhynchus hyacinthinus)
|
|---|---|
| Published in |
Genetics and Molecular Biology, January 2013
|
| DOI | 10.1590/s1415-47572013005000001 |
| Pubmed ID | |
| Authors |
Flavia T. Presti, Janaína Meyer, Paulo T.Z. Antas, Neiva M.R. Guedes, Cristina Y. Miyaki |
| Abstract |
Molted feather sampling is a useful tool for genetic analyses of endangered species, but it is often very laborious due to the low quality and quantity of the DNA obtained. In the present study we show the parts of feathers that resulted in better yield of DNA. In descending order these were: blood clot outside the umbilicus, umbilicus (without blood clot), tip, inner membrane, and small calamus. Compared to DNA extracted from blood samples, DNA extracted from feathers produced microsatellite alleles of poorer quality and had to be processed immediately after extraction. As expected due to the level of DNA degradation, molecular sexing protocols that result in shorter PCR products were more efficient. |
Mendeley readers
The data shown below were compiled from readership statistics for 59 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 2% |
| Australia | 1 | 2% |
| Brazil | 1 | 2% |
| Unknown | 56 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 16 | 27% |
| Student > Ph. D. Student | 9 | 15% |
| Researcher | 8 | 14% |
| Student > Bachelor | 7 | 12% |
| Professor | 4 | 7% |
| Other | 9 | 15% |
| Unknown | 6 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 33 | 56% |
| Environmental Science | 6 | 10% |
| Biochemistry, Genetics and Molecular Biology | 5 | 8% |
| Veterinary Science and Veterinary Medicine | 4 | 7% |
| Psychology | 2 | 3% |
| Other | 2 | 3% |
| Unknown | 7 | 12% |