Title |
Enhancement of chitosanase production by cell immobilization of Gongronella sp. JG
|
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Published in |
Brazilian Journal of Microbiology, January 2013
|
DOI | 10.1590/s1517-83822013005000017 |
Pubmed ID | |
Authors |
Pingping Zhang, Wei Zhou, Peng Wang, Li Wang, Mingli Tang |
Abstract |
Chitosanase production of Gongronella sp. JG cells immobilized in calcium alginate gel and polyurethane foam was compared with that of the free cells, there was a 60% increase in the enzyme yield (2429 U/L) compared to the highest yield obtained from free cells (1513 U/L). The optimal immobilization parameters (concentrations of sodium alginate, calcium chloride, bead inoculums, bead diameter, etc) for the enhanced production of chitosanase were determined as: sodium alginate 2% (w/v), 0.1 M calcium chloride, inoculum 10 mL beads to 100 mL production media and 2.7 mm bead diameter. Maximum chitosanase production was achieved with initial pH of 5.5 and temperature of 30 °C. The alginate beads had well stability, retained 85% ability of enzyme production even after 7 cycles of repeated batch fermentation. These results showed the immobilization technique was a feasible and economical method for chitosansase production by Gongronella sp. JG. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 12 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 3 | 25% |
Professor | 1 | 8% |
Student > Bachelor | 1 | 8% |
Student > Ph. D. Student | 1 | 8% |
Researcher | 1 | 8% |
Other | 0 | 0% |
Unknown | 5 | 42% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 17% |
Agricultural and Biological Sciences | 2 | 17% |
Chemical Engineering | 1 | 8% |
Engineering | 1 | 8% |
Unknown | 6 | 50% |