Title |
KPC-2-producing Klebsiella pneumoniae in a hospital in the Midwest region of Brazil
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Published in |
Brazilian Journal of Microbiology, June 2015
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DOI | 10.1590/s1517-838246246220140174 |
Pubmed ID | |
Authors |
Camila Arguelo Biberg, Ana Claudia Souza Rodrigues, Sidiane Ferreira do Carmo, Claudia Elizabeth Volpe Chaves, Ana Cristina Gales, Marilene Rodrigues Chang |
Abstract |
The emergence of β-lactamase-producing Enterobacteriaceae in the last few decades has become major challenge faced by hospitals. In this study, isolates of Klebsiella pneumoniae carbapenemase-2 (KPC-2)-producing K. pneumoniae from a tertiary hospital in Mato Grosso do Sul, Brazil, were characterized. Bacterial identification was performed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF; Bruker Daltonics, Germany) mass spectrometry. The minimum inhibitory concentrations of carbapenems were determined using the agar dilution method as recommended by the Clinical Laboratory Standards Institute guidelines. Carbapenemase production was detected using the modified Hodge test (MHT) and polymerase chain reaction (PCR), followed by DNA sequencing. Of 360 (12.2%) K. pneumoniae isolates obtained between May 2009 and May 2010, 44 (12.2%) were carbapenem nonsusceptible. Of these 44 isolates, thirty-six K. pneumoniae isolates that were positive by MHT and PCR carried the bla KPC-2 gene. Thus, KPC-2producing Klebsiella pneumoniae has been present in a Brazilian hospital located in the Midwest region since at least 2009. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 33% |
Australia | 1 | 33% |
Unknown | 1 | 33% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 1 | 1% |
Unknown | 70 | 99% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 15 | 21% |
Researcher | 13 | 18% |
Student > Bachelor | 12 | 17% |
Student > Doctoral Student | 4 | 6% |
Student > Postgraduate | 3 | 4% |
Other | 8 | 11% |
Unknown | 16 | 23% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 12 | 17% |
Immunology and Microbiology | 10 | 14% |
Biochemistry, Genetics and Molecular Biology | 9 | 13% |
Agricultural and Biological Sciences | 8 | 11% |
Nursing and Health Professions | 5 | 7% |
Other | 11 | 15% |
Unknown | 16 | 23% |