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Identification of Cryptosporidiumspecies and genotypes in dairy cattle in Brazil

Overview of attention for article published in Revista Brasileira de Parasitologia Veterinária, March 2013
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Title
Identification of Cryptosporidiumspecies and genotypes in dairy cattle in Brazil
Published in
Revista Brasileira de Parasitologia Veterinária, March 2013
DOI 10.1590/s1984-29612013005000010
Pubmed ID
Authors

Flavio Medeiros Paz e Silva, Raimundo Souza Lopes, João Pessoa Araújo-Junior

Abstract

In this study, we identified Cryptosporidium species and genotypes present in dairy cattle in the central region of São Paulo state, Brazil. Fecal specimens were collected from 200 animals (100 calves and 100 cows) in ten dairy farms. Fecal samples were examined using microscopic examination (ME), enzyme immunoassay (EIA) and polymerase chain reaction (PCR). Cryptosporidium species and genotypes were determined by restriction fragment length polymorphism (RFLP) or DNA sequencing analysis of the SSU-rRNA and GP60 genes. The occurrence of Cryptosporidium spp. infection was 14% (28/200). The occurrence in calves (26%) was significantly higher than in cows (2%). Of the 27 Cryptosporidium-positive specimens submitted to genotyping, C. andersoni was identified in 23 (85.1%), C. bovis in three (11.1%), and the zoonotic C. parvum subtype IIaA15G2R1 in one (3.7%). The study demonstrates that Cryptosporidium spp. infection was common and widespread in dairy cattle in this region and that calves have a high prevalence of C. andersoni. Furthermore, the presence of C. parvum subtype IIaA15G2R1 indicates that dairy calves from this region should be considered a potential source of zoonotic Cryptosporidium oocysts.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 44 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 44 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 11 25%
Student > Ph. D. Student 6 14%
Student > Doctoral Student 5 11%
Researcher 5 11%
Student > Postgraduate 3 7%
Other 7 16%
Unknown 7 16%
Readers by discipline Count As %
Agricultural and Biological Sciences 14 32%
Veterinary Science and Veterinary Medicine 10 23%
Medicine and Dentistry 4 9%
Biochemistry, Genetics and Molecular Biology 3 7%
Environmental Science 2 5%
Other 4 9%
Unknown 7 16%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 February 2015.
All research outputs
#22,759,452
of 25,373,627 outputs
Outputs from Revista Brasileira de Parasitologia Veterinária
#432
of 660 outputs
Outputs of similar age
#184,888
of 210,225 outputs
Outputs of similar age from Revista Brasileira de Parasitologia Veterinária
#17
of 21 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 660 research outputs from this source. They receive a mean Attention Score of 1.2. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 21 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.