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Prolonged viability of human organotypic skin explant in culture method (hOSEC)

Overview of attention for article published in Anais Brasileiros de Dermatologia, June 2015
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Title
Prolonged viability of human organotypic skin explant in culture method (hOSEC)
Published in
Anais Brasileiros de Dermatologia, June 2015
DOI 10.1590/abd1806-4841.20153645
Pubmed ID
Authors

Marco Andrey Cipriani Frade, Thiago Antônio Moretti de Andrade, Andréia Fernanda Carvalho Leone Aguiar, Flávia Araújo Guedes, Marcel Nani Leite, Williane Rodrigues Passos, Eduardo Barbosa Coelho, Pranab Kummar Das

Abstract

Currently, the cosmetic industry is overwhelmed in keeping up with the safety assessment of the increasing number of new products entering the market. To meet such demand, research centers have explored alternative methods to animal testing and also the large number of volunteers necessary for preclinical and clinical tests. This work describes the human skin ex-vivo model (hOSEC: Human Organotypic Skin Explant Culture) as an alternative to test the effectiveness of cosmetics and demonstrate its viability through cutaneous keratinocytes' proliferative capacity up to 75 days in culture. The skin explants obtained from surgeries were cultured in CO2-humid incubator. After 1, 7, 30 and 75 days in culture, skin fragments were harvested for analysis with histomorphological exam (HE staining) on all days of follow-up and immunohistochemistry for Ck5/6, Ck10 and Ki-67 only on the 75th day. On the 7th day, the epidermis was perfect in the dermoepidermal junction, showing the viability of the model. On the 30th day, the epidermis was thicker, with fewer layers on the stratum corneum, although the cutaneous structure was unaltered. On the 75th day, the skin became thinner but the dermoepidermal junctions were preserved and epidermal proliferation was maintained. After the 75th day on culture, the skin was similar to normal skin, expressing keratinocytes with Ck5/6 on supra-basal layers; Ck10 on differentiated layers; and viability could be assessed by the positivity of basal cells by Ki-67. The hOSEC model seems a good alternative to animal testing; it can be used as a preclinical test analogous to clinical human skin test with similar effectiveness and viability proven by immunohistological analyses.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 98 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 1%
Brazil 1 1%
Unknown 96 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 21 21%
Researcher 15 15%
Student > Bachelor 12 12%
Student > Master 9 9%
Other 4 4%
Other 14 14%
Unknown 23 23%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 18 18%
Agricultural and Biological Sciences 14 14%
Medicine and Dentistry 11 11%
Engineering 7 7%
Pharmacology, Toxicology and Pharmaceutical Science 6 6%
Other 15 15%
Unknown 27 28%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 June 2015.
All research outputs
#21,157,205
of 25,986,827 outputs
Outputs from Anais Brasileiros de Dermatologia
#5
of 6 outputs
Outputs of similar age
#208,203
of 282,785 outputs
Outputs of similar age from Anais Brasileiros de Dermatologia
#8
of 20 outputs
Altmetric has tracked 25,986,827 research outputs across all sources so far. This one is in the 10th percentile – i.e., 10% of other outputs scored the same or lower than it.
So far Altmetric has tracked 6 research outputs from this source. They receive a mean Attention Score of 1.6. This one scored the same or higher as 1 of them.
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We're also able to compare this research output to 20 others from the same source and published within six weeks on either side of this one. This one is in the 10th percentile – i.e., 10% of its contemporaries scored the same or lower than it.