Title |
A cysteine protease from the latex of Ficus benjamina has in vitro anthelmintic activity against Haemonchus contortus
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Published in |
Revista Brasileira de Parasitologia Veterinária, November 2018
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DOI | 10.1590/s1984-296120180070 |
Pubmed ID | |
Authors |
Ledia Feitosa Wanderley, Alexandra Martins dos Santos Soares, Carolina Rocha e Silva, Isaias Moreira de Figueiredo, Andre Teixeira da Silva Ferreira, Jonas Perales, Handerson Ribeiro de Oliveira Mota, Jose Tadeu Abreu Oliveira, Livio Martins Costa |
Abstract |
Haemonchus contortus is a gastrointestinal nematode that is responsible for high mortality rates in ruminant herds. The resistance of nematodes to synthetic anthelmintics is widespread and requires a continuous search for new bioactive molecules, such as proteins. The objective of this study was to evaluate the anthelmintic potential of a protease purified from the latex of Ficus benjamina against H. contortus . Fresh latex was collected from plants via small incisions in the green stems, the rubber was removed by centrifugation, and the latex protein extract (LPE) was obtained. After LPE fractionation with ammonium sulfate and chromatography of the fraction containing the highest proteolytic activity on CM-cellulose, a cysteine protease (FbP) was purified. FbP has a molecular mass of approximately 23.97 kDa, and its proteolytic activity was stable between pH 6.0 and pH 10 and over a broad temperature range, with optimum activity at 60 °C. FbP inhibited both the development and exsheathment of H. contortus larvae, with 50% effective concentrations of 0.26 and 0.79 mg/mL, respectively. We conclude that this cysteine protease from F. benjamina latex with anthelmintic activity against H. contortus could be a promising alternative for the development of products for use in parasite control programmes. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Brazil | 1 | 50% |
United Kingdom | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Science communicators (journalists, bloggers, editors) | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 29 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 4 | 14% |
Student > Bachelor | 4 | 14% |
Student > Doctoral Student | 2 | 7% |
Other | 2 | 7% |
Researcher | 2 | 7% |
Other | 4 | 14% |
Unknown | 11 | 38% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 4 | 14% |
Pharmacology, Toxicology and Pharmaceutical Science | 4 | 14% |
Veterinary Science and Veterinary Medicine | 2 | 7% |
Environmental Science | 1 | 3% |
Biochemistry, Genetics and Molecular Biology | 1 | 3% |
Other | 4 | 14% |
Unknown | 13 | 45% |