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Detection of Toxoplasma gondii DNA in peripheral blood and aqueous humor of patients with Toxoplasmic active focal necrotizing retinochoroiditis using real-time PCR

Overview of attention for article published in Arquivos Brasileiros de Oftalmologia, January 2015
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Title
Detection of Toxoplasma gondii DNA in peripheral blood and aqueous humor of patients with Toxoplasmic active focal necrotizing retinochoroiditis using real-time PCR
Published in
Arquivos Brasileiros de Oftalmologia, January 2015
DOI 10.5935/0004-2749.20150094
Pubmed ID
Authors

Fabio Felipe dos Santos, Heloisa Nascimento, Cristina Muccioli, Deise Fialho da Costa, Luiz Vicente Rizzo, Alessandra Gonçalves Commodaro, Rubens Belfort

Abstract

To evaluate the ability of real-time quantitative PCR (qPCR) for detectingToxoplasma gondii DNA in the peripheral blood and aqueous humor of patients with toxoplasmic active focal necrotizing retinochoroiditis. Fifty-five patients with infectious uveitis seen from 2009 to 2013 at the Department of Ophthalmology and Visual Sciences of the Federal University of São Paulo were enrolled in this study. Forty-three patients had toxoplasmic active focal necrotizing retinochoroiditis, and the remaining 12 had non-toxoplasmic infectious uveitis and served as controls. qPCR analysis forT. gondii DNA was performed on the patients' peripheral blood and aqueous humor samples. The qPCR was positive for T. gondii DNA in 37.21% (16/43) of the aqueous humor samples and 2.33% (1/43) of the peripheral blood samples; further, 16.27% (7/43) of the patients had positive results in both their blood and aqueous humor samples. qPCR was able to detect T. gondii DNA in patients with toxoplasmic active focal necrotizing retinochoroiditis in the blood as well as the aqueous humor and can help with the diagnosis of the disease.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 42 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 42 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 6 14%
Student > Bachelor 6 14%
Researcher 4 10%
Student > Postgraduate 4 10%
Professor 3 7%
Other 9 21%
Unknown 10 24%
Readers by discipline Count As %
Medicine and Dentistry 7 17%
Biochemistry, Genetics and Molecular Biology 5 12%
Agricultural and Biological Sciences 5 12%
Veterinary Science and Veterinary Medicine 4 10%
Immunology and Microbiology 4 10%
Other 5 12%
Unknown 12 29%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 July 2016.
All research outputs
#20,653,708
of 25,371,288 outputs
Outputs from Arquivos Brasileiros de Oftalmologia
#255
of 446 outputs
Outputs of similar age
#266,628
of 359,525 outputs
Outputs of similar age from Arquivos Brasileiros de Oftalmologia
#16
of 52 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. This one is in the 10th percentile – i.e., 10% of other outputs scored the same or lower than it.
So far Altmetric has tracked 446 research outputs from this source. They receive a mean Attention Score of 2.5. This one is in the 28th percentile – i.e., 28% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 359,525 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 14th percentile – i.e., 14% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 52 others from the same source and published within six weeks on either side of this one. This one is in the 48th percentile – i.e., 48% of its contemporaries scored the same or lower than it.