Title |
Use of TaqMan Array Card for the Detection of Respiratory Viral Pathogens in Children under 5 Years Old Hospitalised with Acute Medical Illness in Ballabgarh, Haryana, India
|
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Published in |
Indian Journal of Medical Microbiology, January 2019
|
DOI | 10.4103/ijmm.ijmm_18_146 |
Pubmed ID | |
Authors |
Bharti Gaur, Siddhartha Saha, A. Danielle Iuliano, Sanjay K. Rai, Anand Krishnan, Seema Jain, Brett Whitaker, Jonas Winchell, Renu B. Lal, Shobha Broor |
Abstract |
Historical specimens collected from hospitalized children were tested for the following 13 viruses: influenza A and B; respiratory syncytial virus (RSV); parainfluenza viruses 1-3; human metapneumovirus; rhinovirus; coronaviruses 229E, OC43, NL63 and HKU1 and Adenovirus using monoplex real-time reverse transcriptase polymerase chain reaction (rRT-PCR). They were retested using TaqMan Array Card (TAC), a micro-fluidic system, capable of simultaneous multi-pathogen testing, to evaluate its sensitivity and specificity against monoplex rRT-PCR. TAC showed high sensitivity (71%-100%) and specificity (98%-100%) for these viruses in comparison to monoplex rRT-PCR. Multi-specimen detection with high sensitivity and specificity makes TAC a potentially useful tool for both surveillance and outbreak investigations. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 36 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 5 | 14% |
Student > Bachelor | 5 | 14% |
Researcher | 4 | 11% |
Student > Postgraduate | 3 | 8% |
Student > Ph. D. Student | 3 | 8% |
Other | 5 | 14% |
Unknown | 11 | 31% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 7 | 19% |
Immunology and Microbiology | 4 | 11% |
Social Sciences | 3 | 8% |
Nursing and Health Professions | 2 | 6% |
Biochemistry, Genetics and Molecular Biology | 2 | 6% |
Other | 6 | 17% |
Unknown | 12 | 33% |