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Detection of TP53/PIK3CA Mutations in Cell-Free Plasma DNA From Metastatic Breast Cancer Patients Using Next Generation Sequencing

Overview of attention for article published in Clinical Breast Cancer, May 2016
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Title
Detection of TP53/PIK3CA Mutations in Cell-Free Plasma DNA From Metastatic Breast Cancer Patients Using Next Generation Sequencing
Published in
Clinical Breast Cancer, May 2016
DOI 10.1016/j.clbc.2016.05.004
Pubmed ID
Authors

Chiaki Nakauchi, Naofumi Kagara, Kenzo Shimazu, Atsushi Shimomura, Yasuto Naoi, Masafumi Shimoda, Seung Jin Kim, Shinzaburo Noguchi

Abstract

Circulating tumor DNA (ctDNA) within a liquid biopsy is a promising marker for genotyping metastatic tumors. We performed next generation whole exon sequencing of TP53 and PIK3CA genes, which are the 2 most common genetic alterations in breast cancer, in plasma DNA (pDNA) of 17 metastatic breast cancer (MBC) patients and in tumor DNA (tDNA) from their primary tumors. We identified 11 mutations (6 in TP53 and 5 in PIK3CA) in tDNA from 8 patients (47%) and 13 mutations (6 in TP53 and 7 in PIK3CA) in pDNA from 7 patients (41%). Six mutations in pDNA were also identified in tDNA but seven were not. Six MBC patients with TP53 and/or PIK3CA mutations in pDNA had a significantly worse survival rate (P < .05) after recurrence than that of the other 8 MBC patients without these mutations. Carcinoembryonic antigen and cancer antigen 15-3 levels did not correlate with prognosis (P = .675 and P = .877, respectively). These results suggest that mutations in ctDNA can be detected with next generation sequencing in MBC patients and could be a more useful prognostic factor for survival after recurrence than conventional tumor markers.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 69 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 69 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 9 13%
Student > Ph. D. Student 8 12%
Student > Bachelor 7 10%
Other 6 9%
Student > Master 5 7%
Other 11 16%
Unknown 23 33%
Readers by discipline Count As %
Medicine and Dentistry 19 28%
Biochemistry, Genetics and Molecular Biology 13 19%
Agricultural and Biological Sciences 4 6%
Neuroscience 2 3%
Computer Science 1 1%
Other 7 10%
Unknown 23 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 April 2019.
All research outputs
#16,048,009
of 25,374,917 outputs
Outputs from Clinical Breast Cancer
#524
of 1,061 outputs
Outputs of similar age
#183,891
of 328,712 outputs
Outputs of similar age from Clinical Breast Cancer
#9
of 34 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. This one is in the 34th percentile – i.e., 34% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,061 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.5. This one is in the 47th percentile – i.e., 47% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 328,712 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 41st percentile – i.e., 41% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 34 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.