| Title |
Multi-center evaluation of one commercial and 12 in-house real-time PCR assays for detection of Mycoplasma pneumoniae
|
|---|---|
| Published in |
Diagnostic Microbiology & Infectious Disease, March 2017
|
| DOI | 10.1016/j.diagmicrobio.2017.03.004 |
| Pubmed ID | |
| Authors |
Roger Dumke, Alvaro J. Benitez, Victoria Chalker, Karolina Gullsby, Birgit Henrich, Carlos Hidalgo-Grass, Theo Hoogenboezem, Darja Kese, Katherine Loens, Jolanda Maaskant, Ayelet Michael-Gayego, Allon E. Moses, Ran Nir-Paz, Suzan D. Pas, Sabine Pereyre, Randi F. Petersen, Meike Rosenblatt, Annemarie M.C. van Rossum, Søren A. Uldum, Wendy W.J. Unger, Dominique Ursi, Jonas M. Winchell, Cecile Bebear, on behalf of ESCMID Study Group for Mycoplasma Infections |
| Abstract |
Detection of Mycoplasma pneumoniae by real-time PCR is not yet standardized across laboratories. We have implemented a standardization protocol to compare the performance of thirteen commercial and in-house approaches. Despite differences on threshold values of samples, all assays were able to detect at least 20M. pneumoniae genomes per reaction. |
Mendeley readers
The data shown below were compiled from readership statistics for 31 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 31 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Other | 6 | 19% |
| Researcher | 4 | 13% |
| Professor > Associate Professor | 4 | 13% |
| Student > Master | 4 | 13% |
| Lecturer > Senior Lecturer | 2 | 6% |
| Other | 6 | 19% |
| Unknown | 5 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 7 | 23% |
| Immunology and Microbiology | 7 | 23% |
| Agricultural and Biological Sciences | 4 | 13% |
| Medicine and Dentistry | 3 | 10% |
| Veterinary Science and Veterinary Medicine | 2 | 6% |
| Other | 2 | 6% |
| Unknown | 6 | 19% |