Chapter 1 Cotranscriptional Assembly and Native Purification of Large RNA–RNA Complexes for Structural Analyses

Chapter 2 Probing RNA Structures and Interactions Using Fluorescence Lifetime Analyses

Chapter 3 Characterizing Fluorescence Properties of Turn-on RNA Aptamers

Chapter 5 Isothermal Titration Calorimetry Analysis of a Cooperative Riboswitch Using an Interdependent-Sites Binding Model

Chapter 6 Application of Molecular Dynamics to Expand Docking Program’s Exploratory Capabilities and to Evaluate Its Predictions

Chapter 7 Exploring the Binding Process of Cognate Ligand to Add Adenine Riboswitch Aptamer by Using Explicit Solvent Molecular Dynamics (MD) Simulation

Chapter 8 Prediction of Dynamic RBP–RNA Interactions Using PrismNet

Chapter 9 High-Resolution Atomic Force Microscopy Imaging of RNA Molecules in Solution

Chapter 10 3D Structural Analysis of Long Noncoding RNA by Small Angle X-ray Scattering and Computational Modeling

Chapter 11 Combining Biophysical Methods for Structure–Function Analyses of RNA in Solution

Chapter 12 Near-Atomic Resolution Cryo-EM Image Reconstruction of RNA

Chapter 13 Auto-DRRAFTER: Automated RNA Modeling Based on Cryo-EM Density

Chapter 14 X-Ray Crystallography to Study Conformational Changes in a TPP Riboswitch

Chapter 15 Preparation of RNA Microcrystals for Serial Femtosecond Crystallography Experiments

Chapter 16 Mix-and-Inject Serial Femtosecond Crystallography to Capture RNA Riboswitch Intermediates