| Title |
Proteomics Analysis Reveals Stable Multiprotein Complexes in Both Fission and Budding Yeasts Containing Myb-Related Cdc5p/Cef1p, Novel Pre-mRNA Splicing Factors, and snRNAs
|
|---|---|
| Published in |
Molecular & Cellular Biology, March 2023
|
| DOI | 10.1128/mcb.22.7.2011-2024.2002 |
| Pubmed ID | |
| Authors |
Melanie D. Ohi, Andrew J. Link, Liping Ren, Jennifer L. Jennings, W. Hayes McDonald, Kathleen L. Gould |
| Abstract |
Schizosaccharomyces pombe Cdc5p and its Saccharomyces cerevisiae ortholog, Cef1p, are essential Myb-related proteins implicated in pre-mRNA splicing and contained within large multiprotein complexes. Here we describe the tandem affinity purification (TAP) of Cdc5p- and Cef1p-associated complexes. Using transmission electron microscopy, we show that the purified Cdc5p complex is a discrete structure. The components of the S. pombe Cdc5p/S. cerevisiae Cef1p complexes (termed Cwfs or Cwcs, respectively) were identified using direct analysis of large protein complex (DALPC) mass spectrometry (A. J. Link et al., Nat. Biotechnol. 17:676-682, 1999). At least 26 proteins were detected in the Cdc5p/Cef1p complexes. Comparison of the polypeptides identified by S. pombe Cdc5p purification with those identified by S. cerevisiae Cef1p purification indicates that these two yeast complexes are nearly identical in composition. The majority of S. pombe Cwf proteins and S. cerevisiae Cwc proteins are known pre-mRNA splicing factors including core Sm and U2 and U5 snRNP components. In addition, the complex contains the U2, U5, and U6 snRNAs. Previously uncharacterized proteins were also identified, and we provide evidence that several of these novel factors are involved in pre-mRNA splicing. Our data represent the first comprehensive analysis of CDC5-associated proteins in yeasts, describe a discrete highly conserved complex containing novel pre-mRNA splicing factors, and demonstrate the power of DALPC for identification of components in multiprotein complexes. |
Mendeley readers
The data shown below were compiled from readership statistics for 96 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 1% |
| Canada | 1 | 1% |
| Unknown | 94 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 21 | 22% |
| Researcher | 21 | 22% |
| Student > Bachelor | 7 | 7% |
| Student > Master | 7 | 7% |
| Student > Doctoral Student | 6 | 6% |
| Other | 17 | 18% |
| Unknown | 17 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 46 | 48% |
| Biochemistry, Genetics and Molecular Biology | 26 | 27% |
| Immunology and Microbiology | 2 | 2% |
| Medicine and Dentistry | 2 | 2% |
| Social Sciences | 1 | 1% |
| Other | 1 | 1% |
| Unknown | 18 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 July 2018.
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#8,588,963
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Outputs from Molecular & Cellular Biology
#4,297
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#150,792
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Outputs of similar age from Molecular & Cellular Biology
#3,253
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